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chemidoc xsr molecular imager  (Bio-Rad)


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    Structured Review

    Bio-Rad chemidoc xsr molecular imager
    Chemidoc Xsr Molecular Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chemidoc+xsr+molecular+imager/chemidoc+mp+imaging+system/pm37865314-184-30-34
    Average 90 stars, based on 1 article reviews
    chemidoc xsr molecular imager - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: Reciprocal Regulation of Notch and Nuclear Factor of Activated T-cells (NFAT) c1 Transactivation in Osteoblasts
    Article Snippet: Membranes were blocked with 3% bovine serum albumin (BSA) in PBS and exposed to a 1:1000 dilution of a rabbit polyclonal antibody to NICD (Cell Signaling Technology), Hey1 (Santa Cruz), or Hey2 (Abcam, Cambridge, UK), to a 1:1000 dilution of murine monoclonal antibody to NFATc1 (Santa Cruz Biotechnology), or to a 1:1000 dilution of goat polyclonal antibody to CSL (Santa Cruz Biotechnology). .. All blots were exposed to anti-rabbit, anti-mouse, or anti-goat IgG conjugated to horseradish peroxidase (Sigma), incubated with a chemiluminescence detection reagent (Bio-Rad) and acquired with a cooled charged coupled device camera mounted on the Chemidoc XSR molecular imager (Bio-Rad). .. To assess even loading of the samples, antibodies were removed by washing in 70 m m glycine, 0.1% SDS, and 1% Tween 20 (Sigma), pH 2.2, followed by rinsing with PBS and 0.1% Tween 20 in PBS.

    Article Title: Reciprocal Regulation of Notch and Nuclear Factor of Activated T-cells (NFAT) c1 Transactivation in Osteoblasts
    Article Snippet: Membranes were blocked with 3% bovine serum albumin (BSA) in PBS and exposed to a 1:1000 dilution of a rabbit polyclonal antibody to NICD (Cell Signaling Technology), Hey1 (Santa Cruz), or Hey2 (Abcam, Cambridge, UK), to a 1:1000 dilution of murine monoclonal antibody to NFATc1 (Santa Cruz Biotechnology), or to a 1:1000 dilution of goat polyclonal antibody to CSL (Santa Cruz Biotechnology). .. All blots were exposed to anti-rabbit, anti-mouse, or anti-goat IgG conjugated to horseradish peroxidase (Sigma), incubated with a chemiluminescence detection reagent (Bio-Rad) and acquired with a cooled charged coupled device camera mounted on the Chemidoc XSR molecular imager (Bio-Rad). ..

    Article Title: Nuclear Factor of Activated T-cells (NFAT)c2 Inhibits Notch Receptor Signaling in Osteoblasts
    Article Snippet: A rabbit polyclonal antibody raised against the N terminus of NFATc2 (antibody 4389, Cell Signaling Technologies) was used for the detection of endogenous NFATc2, whereas a mouse monoclonal antibody (clone 25A10.D6.D2, Thermo Scientific) was used for the detection of caNFATc2. .. Blots were exposed to either anti-rabbit IgG or anti-mouse IgG conjugated to horseradish peroxidase (Sigma-Aldrich) and incubated with a chemiluminescence detection reagent (Bio-Rad), and digital images were acquired with a cooled charged coupled device camera, mounted on the Chemidoc XSR molecular imager (Bio-Rad). .. To assess even loading of the samples, membranes were stripped and reprobed with a 1:1000 dilution of a goat polyclonal antibody against actin (I-19, Santa Cruz Biotechnology, Santa Cruz, CA) and exposed to anti-goat IgG conjugated to horseradish peroxidase, and digital images were acquired with a Chemidoc XSR molecular imager.

    Article Title: Notch Suppresses Nuclear Factor of Activated T Cells (Nfat) Transactivation and Nfatc1 Expression in Chondrocytes
    Article Snippet: Membranes were blocked with 3% BSA in PBS and exposed to a 1:1000 dilution of murine monoclonal antibody to Nfatc1 (7A6; Santa Cruz Biotechnology, Santa Cruz, California), and to assess even loading of the samples, membranes were exposed to a 1:1000 dilution of a goat polyclonal antibody against actin (I-19; Santa Cruz Biotechnology). .. All blots were exposed to either antimouse or antigoat IgG conjugated to horseradish peroxidase, incubated with a chemiluminescence detection reagent (Bio-Rad), and acquired with a cooled charge-coupled device camera mounted on a Chemidoc XSR molecular imager (Bio-Rad). ..

    Article Title: Nuclear Factor of Activated T-cells (NFAT)c2 Inhibits Notch Receptor Signaling in Osteoblasts
    Article Snippet: A rabbit polyclonal antibody raised against the N terminus of NFATc2 (antibody 4389, Cell Signaling Technologies) was used for the detection of endogenous NFATc2, whereas a mousemonoclonal antibody (clone 25A10.D6.D2, Thermo Scientific) was used for the detection of caNFATc2. .. Blots were exposed to either anti-rabbit IgG or anti-mouse IgG conjugated to horseradish peroxidase (Sigma-Aldrich) and incubated with a chemiluminescence detection reagent (Bio-Rad), and digital images were acquired with a cooled charged coupled device camera, mounted on the Chemidoc XSR molecular imager (Bio-Rad). .. To assess even loading of the samples, membranes were stripped and reprobed with a 1:1000 dilution of a goat polyclonal antibody against actin (I-19, Santa Cruz Biotechnology, Santa Cruz, CA) and exposed to anti-goat IgG conjugated to horseradish peroxidase, and digital images were acquired with a Chemidoc XSR molecular imager.



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